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Published on January 28, 2024

When Is Saliva Hormone Test Done And What Are Its Uses?

Saliva hormone testing has grown in popularity as an easy, informative, and non-invasive method of evaluating hormone levels in the body. As per experts like Healthy Remedies, with a simple spit into a collection tube, levels of key hormones like cortisol, testosterone, estrogen, progesterone, and others can be effectively measured. But when exactly should saliva hormone test be utilized and why are they advantageous?

What is Saliva Hormone Testing?

Saliva hormone testing measures the levels of different hormones present in a spit sample provided by an individual. Typically collection tubes are used which are filled with saliva by spitting into them. These samples are then sent off to a laboratory for analysis. The levels of hormones like cortisol, testosterone, estrogen, progesterone, melatonin, and others can be read to provide a snapshot of what is occurring hormonally in that person’s body at the time. Because hormones show variability and fluctuations throughout days, being able to collect and test saliva is useful for understanding their patterns.

When Might Saliva Hormone Testing Be Ordered? 

Doctors and healthcare providers may decide saliva hormone testing should be ordered for a number of different reasons pertaining to an individual’s symptoms or health status, including:

Investigating General Symptoms of Hormonal Imbalance: Nonspecific symptoms like fatigue, trouble sleeping, hot flashes, changes in libido or appetite could potentially stem from issues with hormones that show up in saliva testing.

Fertility and Reproductive Health Monitoring: For couples attempting to get pregnant, saliva provides details on ovulation timing by tracking reproductive hormones like estrogen and progesterone. It also aids in evaluation of conditions causing infertility.

Wellness Screening and Preventative Care: Saliva hormone levels provide broad insights even for healthy adults into how everyday lifestyle factors are influencing endocrine health and function.

Cancer Treatment Monitoring: Certain cancers, like breast and prostate cancer, have treatments meant to alter hormone levels – saliva testing enables easy monitoring of patient levels during treatment regimens. 

What Are the Major Benefits of Saliva Testing?

As a testing method, saliva offers numerous advantages over the commonly used blood serum testing including:

Greater Patient Comfort/Convenience: Saliva collection is non-invasive, painless, simple and can even be done by patients in their own homes before shipping samples off. This also helps substantially lower costs.

Better Assessment of Variability: Hormone levels naturally fluctuate all throughout the day. Saliva looks at the “free” active hormones at a specific moment in time unlike blood. This fluctuation can be important for some conditions. Testing saliva multiple times daily for days enables unique insight into hormone variability.

Usefulness for All Age Groups: Easy, non-invasive saliva collection is feasible for children, adults, and elderly patients alike with only a spit needed. Blood draws can be significantly more challenging, especially in young kids.

Suitability for Repeated Testing: Collecting saliva is relatively easy, comfortable and inexpensive to repeat on a frequent basis allowing tracking of hormone trends over time (like over a full menstrual cycle)


As research continues to validate and inform practical uses, salivary analysis of hormones will likely take on an even bigger role in endocrine system assessment, cancer treatment decisions, athletic performance, and general wellness management. Patients and doctors alike can be empowered by the unique data it provides conveniently and comfortably.

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